Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis

dc.creatorCatenaccio, Alejandra [Ctr Integrat Biol, Fac Sci, Universidad Mayor, Chile]
dc.creatorCourt, Felipe A. [Ctr Integrat Biol, Fac Sci, Universidad Mayor, Chile]
dc.date.accessioned2021-02-03T19:28:20Z
dc.date.accessioned2022-10-18T18:42:55Z
dc.date.available2021-02-03T19:28:20Z
dc.date.available2022-10-18T18:42:55Z
dc.date.created2021-02-03T19:28:20Z
dc.date.issued2018
dc.identifierCatenaccio, A., & Court, F. A. (2018). Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis. Methods in molecular biology (Clifton, N.J.), 1739, 329–337. https://doi.org/10.1007/978-1-4939-7649-2_21
dc.identifier1940-6029
dc.identifiereISSN: 1064-3745
dc.identifierhttp://repositorio.umayor.cl/xmlui/handle/sibum/7337
dc.identifierhttps://pubmed.ncbi.nlm.nih.gov/29546717/
dc.identifierhttps://doi.org/10.1007/978-1-4939-7649-2_21
dc.identifierhttps://link.springer.com/protocol/10.1007%2F978-1-4939-7649-2_21
dc.identifier10.1007/978-1-4939-7649-2_21
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4455121
dc.description.abstractGlial cells regulate a wide variety of neuronal functions during physiological and pathological conditions. Therefore, the study of glial cells and their association with axons is of paramount importance in order to understand the physiology of the nervous system. This chapter describes a detailed protocol to prepare and stain teased nerve fibers from peripheral nerves using fluorescent indirect immunolabeling and staining with vital dyes. For immunofluorescence analysis, we describe techniques to study the axonal compartment and the expression of cytoplasmic and plasma membrane proteins in Schwann cells.
dc.languageen_US
dc.publisherHumana Press
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceMethods in molecular biology (Clifton, N.J.), 1739, 329–337
dc.subjectImmunofluorescence
dc.subjectNerve fiber
dc.subjectPeripheral nervous system
dc.subjectSchwann cell
dc.subjectTeased fibers
dc.titleTeased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis
dc.typeArtículos de revistas


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