This work is about the fabrication of composite thin films of nanoporous silicon/green synthesized silver nanoparticles and their potential use as antibacterial surfaces. To obtain the composite layers, silver nano particles were synthesized using leaf extracts of P. boldus , and subsequently integrated on nanoporous silicon matrix. The achieved samples were characterized microscopically (TEM and AFM), and physiochemically (UV-vis spectroscopy, LIBS, and XRD). Moreover, the antibacterial activity of the composite layers was studied using different strains of Gram-positive (S. aureus and S. epidermidis) and Gram-negative (K. pneumoniae , E. coli , and P. aeruginosa) bacteria. The experimental results showed the potential of these composite thin films as antibacterial surfaces for biomedical applications.