Artículos de revistas
Size of heparin-imprinted nanoparticles reflects the matched interactions with the target molecule
El tamaño de las nanopartículas impresas en heparina refleja las interacciones con la molécula Diana
Registro en:
Sensors 2019, 19, 2415
1424-8220
Autor
Yoshimi, Yasuo
Oino, Daichi
Ohira, Hirofumi
Muguruma, Hitoshi
Moczko, Ewa
Piletsky, Sergey A.
Resumen
It has been shown that the faradic current at an electrode grafted with molecularly imprinted
polymer (MIP) is sensitive to the specific target molecule used as the template. This phenomenon
is applicable to sensors with very high selectivity, but the sensing mechanism is still a black
box. We investigated the size sensitivity of nanoparticles of molecularly imprinted polymers
(MIP-NPs) to a specific interaction for determination of the mechanism of the gate effect and its
feasibility for new applications. Nanoparticles of poly(methacryloxy ethyl trimethylammonium
chloride-co-acrylamide-co-methylenebisacrylamide) imprinted with heparin immobilized on glass
beads were synthesized. The diameter of the MIP-NPs of heparin was increased by the presence of
the heparin template but was insensitive to chondroitin sulfate C (CSC), the analogue of heparin.
The high selectivity of the MIP-NPs was consistent with the selectivity of electrodes grafted with
a heparin-imprinted polymer in our previous studies. The quartz crystal microbalance probes
immobilizing heparin or CSC were sensitive to MIP-NPs, which indicates that the binding ability of
MIP-NP does not discriminate between the template and other glycosaminoglycans. These results
indicate that the size of the MIP-NP is sensitive to the matched binding with the template through the
imprinted cavity.