Oviducal glands throughout the gonad development stages: a case study of Octopus mimus (Cephalopoda)

Abstract

The oviducal glands (ODG) play a crucial role in octopus reproduction. Herein, structural changes of each section of the ODG of Octopus mimus are described histologically throughout the gonad development stages (GDS). To do this, the epithelial height, stereociliated or non-stereociliated epithelium, nucleus type (pycnotic or non-pycnotic), epithelial secretions and the value range of the macroscopic maturity index (MaMI), which directly involves ODG status, were measured. The ODG are internally constituted of two glandular units (central and peripheral glands) and one set of receptacles (the spermathecae). High epithelia (40 to 80 μm) were observed in both gland units during periods with low MaMI values (< 0.1) corresponding to III-mature and IV-pre-spawning. The stereociliated epithelium was only apparent in II-maturing and III-mature in both gland units. The nuclei were noticeably pycnotic in the central gland during III-mature, IV-pre-spawning and Vspawning, but pycnotic in the peripheral gland only during VI-depletion. The epithelium was disorganised during VI-depletion, while sulphated acid mucin was only present during III-mature in the central gland. The epithelium transformations during the GDS are related to the functions of the gland units and to their multiple secretions. The ODG histology complements the GDS and provides better reproductive status assessment.