info:eu-repo/semantics/article
Precipitation of chymotrypsin from fresh bovine pancreas using i-carrageenan
Fecha
2012-12Registro en:
Woitovich Valetti, Nadia; Lombardi, Julia; Boeris, Valeria; Picó, Guillermo Alfredo; Precipitation of chymotrypsin from fresh bovine pancreas using i-carrageenan; Elsevier; Process Biochemistry; 47; 12; 12-2012; 2570-2574
1359-5113
1359-5113
CONICET Digital
CONICET
Autor
Woitovich Valetti, Nadia
Lombardi, Julia
Boeris, Valeria
Picó, Guillermo Alfredo
Resumen
The separation of chymotrypsin from a crude filtrate of fresh bovine pancreas homogenate was carried out using precipitation with a commercially available negatively charged natural strong polyelectrolyte: -carrageenan. The zymogen form of the enzyme was activated by addition of trypsin (0.0001%, w/w), then, the enzyme was precipitated by polyelectrolyte addition at pH 4.50. The non-soluble complex was separated by simple centrifugation and re-dissolved by a pH change to 8.20. The recovery of chymotrypsin biological activity was 60% of the initial activity in the homogenate with 3-fold increase in its specific activity. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times.