info:eu-repo/semantics/article
Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach
Fecha
2019-08Registro en:
Konda, Prathyusha; Murphy, J. Patrick; Nielsen, Morten; Gujar, Shashi; Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach; Humana Press; Methods in Molecular Biology; 2024; 2; 8-2019; 301-307
978-1-4939-9596-7
1064-3745
1940-6029
CONICET Digital
CONICET
Autor
Konda, Prathyusha
Murphy, J. Patrick
Nielsen, Morten
Gujar, Shashi
Resumen
MHC-bound peptide ligands dictate the activation and specificity of CD8+ T- cells-based and thus are important for devising T-cell immunotherapies. In recent times, advances in mass spectrometry (MS) have enabled the precise identification of these peptides, wherein MS/MS spectra are compared against a reference proteome. Unfortunately, matching immunopeptide MS/MS to reference proteome databases is hindered by inflated search spaces attributed to the number of matches that need to be considered due to a lack of enzyme restriction. These large search spaces limit the efficiency with which MHC-I peptides are identified. Here we offer a solution to this problem whereby we describe a targeted database search approach and accompanying tool SpectMHC that is based on a priori predicted MHC-I peptides (Murphy et al., J Proteome Res 16:1806–1816, 2017).