info:eu-repo/semantics/article
ODF1, sperm flagelar protein is expressed in kidney collecting ducts of rats
Fecha
2019-12Registro en:
Cabrillana, María Eugenia; Bocanegra, María Victoria; Monclus, Maria de Los Angeles; Saez Lancellotti, Tania Emilce Estefania; Simón, Layla Yamila; et al.; ODF1, sperm flagelar protein is expressed in kidney collecting ducts of rats; Elsevier; Heliyon; 5; 12; 12-2019; 1-8
2405-8440
CONICET Digital
CONICET
Autor
Cabrillana, María Eugenia
Bocanegra, María Victoria
Monclus, Maria de Los Angeles
Saez Lancellotti, Tania Emilce Estefania
Simón, Layla Yamila
Funes, Abi Karenina
Colombo, Regina Lucía
Ruiz Estrabón, Martín Andres
Vincenti, Amanda Edith
Oliva Virgili, Rafael
Fornes, Miguel Walter
Resumen
ODF1 has been described as an exclusively expressed testicular protein and is located in the outer dense fibersalong the sperm tail. ODF1 has been involved in the sperm motility and in the development of the flagellum, butthe function of ODF1 is not already clear. Other ODF proteins, such as ODF2 have been characterized in othertissues like the basal body of the kidney primary cilium, but so far only the mRNA of ODF1 has been described inother tissues. These observations let us to hypothesize that the expression of the protein ODF1 could not belimited to the testis. Therefore, in the present work we proposed to evaluate if the ODF1 protein could also bepresent in tissues other than the testis. Here we demonstrated through western blot, immunofluorescence, and RTPCR techniques that the protein and mRNA of ODF1 have been identified in the rat kidney. Finally, the presenceof ODF1 in kidney has also been confirmed through proteomic analysis using mass spectrometry. The resultsderived from these different complementary approaches indicate that, to our knowledge and for the first time,ODF1 is demonstrated to be present in an additional organ different to testis. This results raise new questionsabout potential other functions and locations of the ODF1 protein.