info:eu-repo/semantics/article
Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
Fecha
2006-05Registro en:
Rocchetta, Iara; Mazzuca, Marcia; Conforti, Visitacion Teresa D.; Ruiz, Laura Beatriz; Balzaretti, Vilma Teresa; et al.; Effect of chromium on the fatty acid composition of two strains of Euglena gracilis; Elsevier; Environmental Pollution; 141; 2; 5-2006; 353-358
0269-7491
CONICET Digital
CONICET
Autor
Rocchetta, Iara
Mazzuca, Marcia
Conforti, Visitacion Teresa D.
Ruiz, Laura Beatriz
Balzaretti, Vilma Teresa
Rios, Maria del Carmen
Resumen
The effect of hexavalent chromium on fatty acid composition was studied in two strains of Euglena gracilis; UTEX 753 (from the Culture Collection of Algae of Texas University, USA) and MAT (isolated from a highly polluted River). Both were grown in photoauxotrophic and photoheterotrophic conditions and exposed to two metal concentrations, one below and one above IC50. The high malondialdehyde (MDA) levels (3 to 7-fold) obtained with chromium concentration above IC50, suggested the existence of metal-induced lipid peroxidation. Total lipid content increased only with concentration below IC50, whereas it was inhibited by higher metal concentration. Photoheterotrophic control strains exhibited a significantly higher proportion of saturated and polyunsaturated fatty acids. Polyunsaturated acids were most affected by chromium, especially those related to chloroplast structures. Ultra-structure studies showed clear thylakoid disorganization in all treated cells. The results indicate that hexavalent chromium affects levels of fatty acids, especially those related to photosynthetic activity.