info:eu-repo/semantics/article
Deoxythymidine–Pterin Fluorescent Adduct Formation through a Photosensitized Process
Fecha
2017-11Registro en:
Estébanez, Sandra; Thomas, Andrés Héctor; Lorente, Carolina; Deoxythymidine–Pterin Fluorescent Adduct Formation through a Photosensitized Process; Wiley VCH Verlag; Chemphyschem; 19; 3; 11-2017; 300-306
1439-4235
CONICET Digital
CONICET
Autor
Estébanez, Sandra
Thomas, Andrés Héctor
Lorente, Carolina
Resumen
A new fluorescent compound was isolated from UVA-irradiated aqueous solutions containing pterin (Ptr) and 2′deoxythymidine (dT) in anaerobic conditions. Pterins are widespread in living systems in small amounts, but they are accumulated in some pathological situations. Under UVA radiation, pterins are photochemically active, fluorescent, and photosensitize the generation of singlet oxygen [1O2(1Δg)]. The isolated compound was structurally characterized by using liquid chromatography coupled to tandem mass spectrometry, and its photophysical properties were studied with the time-correlated single-photon-counting technique. The molecular weight and the analysis of the fragmentation correspond to a molecule where the pterinic moiety is attached to the thymine nucleobase. The product exhibits photophysical properties similar to those of Ptr, including relatively high fluorescence and 1O2 production quantum yields.