info:eu-repo/semantics/article
Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility
Fecha
2017-05Registro en:
Dudiuk, Catiana Beatriz; Macedo, Daiana; Leonardelli, Florencia; Theill, Laura; Cabeza, Matías Sebastián; et al.; Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility; American Society for Microbiology; Antimicrobial Agents and Chemotherapy; 61; 5; 5-2017; 1-7
0066-4804
CONICET Digital
CONICET
Autor
Dudiuk, Catiana Beatriz
Macedo, Daiana
Leonardelli, Florencia
Theill, Laura
Cabeza, Matías Sebastián
Gamarra, Maria Soledad
Garcia, Guillermo Manuel
Resumen
C. guilliermondii shows intrinsic reduced echinocandin susceptibility. It harbors two polymorphisms (L633M and T634A) at the Fks1p hot-spot 1 region. Our objective was to confirm that C. guilliermondii´s reduced echinocandin susceptibility is due to those naturally occurring substitutions. We constructed a Saccharomyces cerevisiae mutant where a region of its FKS1 gene (including hot-spot 1) was replaced with that from C. guilliermondii The chimeric mutants showed 32-fold echinocandin MIC values increase confirming the hypothesis.