An efficient experimental strategy for mouse embryonic stem cell differentiation and separation of a cytokeratin-19-positive population of insulin-producing cells

Abstract

Embryonic stem cells are a potential source for insulin‐producing cells, but existing differentiation protocols are of limited efficiency. Here, the aim has been to develop a new one, which drives development of embryonic stem cells towards insulin producing cells rather than to neuronal cell types, and to combine this with a strategy for their separation from insulin‐negative cells.