info:eu-repo/semantics/article
17β-Estradiol stimulates the translocation of endogenous estrogen receptor α at the plasma membrane of normal anterior pituitary cells
Fecha
2012-05Registro en:
Gutiérrez, Silvina; Sosa, Liliana del Valle; Petiti, Juan Pablo; Mukdsi, Jorge Humberto; Mascanfroni, Ivan Darío; et al.; 17β-Estradiol stimulates the translocation of endogenous estrogen receptor α at the plasma membrane of normal anterior pituitary cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 355; 1; 5-2012; 169-179
0303-7207
CONICET Digital
CONICET
Autor
Gutiérrez, Silvina
Sosa, Liliana del Valle
Petiti, Juan Pablo
Mukdsi, Jorge Humberto
Mascanfroni, Ivan Darío
Pellizas, Claudia Gabriela
de Paul, Ana Lucia
Cambiaso, Mariana Lujan
Torres, Alicia Ines
Resumen
In the present work we aimed at identifying ERα in the plasma membrane of normal anterior pituitary cells and investigated if 17β-estradiol was able to induce their subcellular redistribution. Our results show that about 8% of anterior pituitary cells expressed ERα in the plasma membrane, with the geometrical mean fluorescence intensity being increased after steroid hormone treatment. 17β-Estradiol and the selective ERα agonist PPT induced an increase of ERα expression in the plasma membrane and activated the PKCα/ERK 1/2 pathway in a time-course not compatible with genomic actions, thus supporting the notion of membrane-initiated effects. These findings suggest that 17β-estradiol stimulates the translocation of endogenous ERα to the plasma membrane, consequently modulating this ER pool and leading to cellular biological effects in normal anterior pituitary gland.