info:eu-repo/semantics/article
PKA isoforms coordinate mRNA fate during nutrient starvation
Fecha
2012-08Registro en:
Tudisca, Vanesa Romina; Simpson, Clare; Castelli, Lydia; Lui, Jennifer; Hoyle, Nathaniel; et al.; PKA isoforms coordinate mRNA fate during nutrient starvation; Company of Biologists; Journal of Cell Science; 125; 21; 8-2012; 5221-5232
0021-9533
CONICET Digital
CONICET
Autor
Tudisca, Vanesa Romina
Simpson, Clare
Castelli, Lydia
Lui, Jennifer
Hoyle, Nathaniel
Moreno, Silvia Margarita
Ashe, Mark
Portela, Paula
Resumen
A variety of stress conditions induce mRNA and protein aggregation into mRNA silencing foci, but the signalling pathways mediating these responses are still elusive. Previously we demonstrated that PKA catalytic isoforms Tpk2 and Tpk3 localise with processing and stress bodies in Saccharomyces cerevisiae. Here, we show that Tpk2 and Tpk3 are associated with translation initiation factors Pab1 and Rps3 in exponentially growing cells. Glucose starvation promotes the loss of interaction between Tpk and initiation factors followed by their accumulation into processing bodies. Analysis of mutants of the individual PKA isoform genes has revealed that the TPK3 or TPK2 deletion affects the capacity of the cells to form granules and arrest translation properly in response to glucose starvation or stationary phase. Moreover, we demonstrate that PKA controls Rpg1 and eIF4G1 protein abundance, possibly controlling cap-dependent translation. Taken together,our data suggest that the PKA pathway coordinates multiple stages in the fate of mRNAs in association with nutritional environment and growth status of the cell. © 2012.