info:eu-repo/semantics/article
Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
Fecha
2017-12Registro en:
Acosta, Cristian Gabriel; Foscolo, Mabel Rosa; Cavicchia, Juan Carlos; Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat; Instituto de Histología y Embriología de Mendoza "Dr. Mario H. Burgos"; Biocell; 41; 2-3; 12-2017; 63-65
0327-9545
1667-5746
CONICET Digital
CONICET
Autor
Acosta, Cristian Gabriel
Foscolo, Mabel Rosa
Cavicchia, Juan Carlos
Resumen
The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine interstice. The former structures generate and release whole cells (exocrine aspect of the gland) while the latter synthesize and release androgens and related hormones. The testis also has poorly understood paracrine connexions. These connexions play an important role during spermatogenesis. A key molecule within these structures is β-actin. Therefore, the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the rat. To achieve this goal, we used a combination of trans-illumination assisted microdissection of seminiferous tubules, confocal immunofluorescence and Western blot analysis. Our experiments revealed that the levels of β-actin fluctuate significantly during spermatogenesis, peaking immediately after spermiation.