info:eu-repo/semantics/article
Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors
Fecha
2018-05-01Registro en:
Mild, Jesica Gabriela; Fernandez, Lucia Raquel; Gayet, Odile; Iovanna, Juan; Dusetti, Nelson; et al.; Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors; Springer; Molecular Biotechnology; 60; 5; 1-5-2018; 369-379
1073-6085
CONICET Digital
CONICET
Autor
Mild, Jesica Gabriela
Fernandez, Lucia Raquel
Gayet, Odile
Iovanna, Juan
Dusetti, Nelson
Edreira, Martin Miguel
Resumen
Chagas disease, a parasitic disease caused by Trypanosoma cruzi, is a major public health burden in poor rural populations of Central and South America and a serious emerging threat outside the endemic region, since the number of infections in non-endemic countries continues to rise. In order to develop more efficient anti-trypanosomal treatments to replace the outdated therapies, new molecular targets need to be explored and new drugs discovered. Trypanosoma cruzi has distinctive structural and functional characteristics with respect to the human host. These exclusive features could emerge as interesting drug targets. In this work, essential and differential protein–protein interactions for the parasite, including the ribosomal P proteins and proteins involved in mRNA processing, were evaluated in a bioluminescence resonance energy transfer-based assay as a starting point for drug screening. Suitable conditions to consider using this simple and robust methodology to screening compounds and natural extracts able to inhibit protein–protein interactions were set in living cells and lysates.