info:eu-repo/semantics/article
CURLY LEAF regulates micro RNA activity by controlling ARGONAUTE 1 degradation in plants
Fecha
2019-10Registro en:
Ré, Delfina Adela; Cambiagno, Damián Alejandro; Arce, Agustín Lucas; Tomassi, Ariel Hernán; Giustozzi, Marisol; et al.; CURLY LEAF regulates micro RNA activity by controlling ARGONAUTE 1 degradation in plants; Oxford University Press; Molecular Plant; 13; 1; 10-2019; 72-87
1674-2052
CONICET Digital
CONICET
Autor
Ré, Delfina Adela
Cambiagno, Damián Alejandro
Arce, Agustín Lucas
Tomassi, Ariel Hernán
Giustozzi, Marisol
Casati, Paula
Ariel, Federico Damian
Manavella, Pablo Andrés
Resumen
CURLY LEAF (CLF) encodes the methyl-transferase sub-unit of the PolycombRepressor Complex 2 (PRC2), which regulates the expression of target genesthrough H3K27 tri-methylation. We isolated a new CLF mutant allele (clf-78)using a genetic screening designed to identify micro RNAs (miRNA) deficientmutants. CLF mutant plants showed impaired miRNA activity caused byincreased AGO1 ubiquitination and enhanced degradation in specific tissues.Such CLF-mediated AGO1 regulation was evidenced when plants wereexposed to UV radiation, causing increased susceptibility of clf mutants to someUV-induced responses. Furthermore, we showed that CLF directly regulatesFBW2, which in turn triggers AGO1 degradation in the mutants. Interestingly,AGO1 bound to a target appeared particularly prone to degradation in themutant plants, a process that is exacerbated when the complex bound a noncleavable target. Thus, a prolonged AGO1-target interaction seems to favorAGO1 degradation, suggesting that non-cleavable miRNA targets mayovercome translation inhibition by modulating AGO1 stability in plants.