info:eu-repo/semantics/article
A natural oil increases specific anti-OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide
Fecha
2020-08Registro en:
Campra, Noelia Anahí; Montironi, Ivana Dalila; Reinoso, Elina Beatríz; Raviolo, José; Ruiz Moreno, Federico Nahuel; et al.; A natural oil increases specific anti-OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide; Federation of American Societies for Experimental Biology; Journal of Leukocyte Biology; 109; 1; 8-2020; 223-232
0741-5400
CONICET Digital
CONICET
Autor
Campra, Noelia Anahí
Montironi, Ivana Dalila
Reinoso, Elina Beatríz
Raviolo, José
Ruiz Moreno, Federico Nahuel
Maletto, Belkys Angélica
Cariddi, Laura Noelia
Resumen
The efficacy of certain vaccines is improved by the use of adjuvants. Nowadays, the development of new, effective, and safe adjuvants that stimulate the innate immune response is researched. In this context, medicinal plants appear as a suitable alternative. Minthostachys verticillata essential oil (EO) has demonstrated the ability to modulate mechanisms of the innate immune response. Thus, the present work aimed to evaluate the EO adjuvant effect on humoral and cellular immunity, coadministered with OVA as antigen. The chemical analysis of EO by gas chromatography–mass spectrometry revealed a predominant pulegone–menthone chemotype. EO (1.25, 2.5, or 5.0 mg/ml) did not alter the viability of murine fibroblasts (3T3 cell line) neither showed signs of toxicity in Balb/c mice inoculated subcutaneously. The serum of mice immunized with OVA + EO showed increased levels of anti-OVA-specific antibodies of IgG1 subclass compared with the mice immunized with OVA alone revealing an adjuvant effect of EO. The delayed type hypersensitivity showed that the combination OVA + Al(OH)3 + EO was the best to induce a cellular immune response that extended until 48 h postinjection of OVA. M. verticillata EO appears as a new, safe, and effective adjuvant, which should continue to be studied for their possible future incorporation into vaccine formulations.