info:eu-repo/semantics/article
Efficacy of topical treatment with (-)-epigallocatechin gallate, a green tea catechin, in mice with cutaneous leishmaniasis
Fecha
2020-04-10Registro en:
Sosa, Andrea Mabel; Moya Alvarez, Agustin; Bracamonte, María Estefanía; Korenaga, Masataka; Marco, Jorge Diego; et al.; Efficacy of topical treatment with (-)-epigallocatechin gallate, a green tea catechin, in mice with cutaneous leishmaniasis; Multidisciplinary Digital Publishing Institute; Molecules; 25; 7; 10-4-2020; 1-9
1420-3049
CONICET Digital
CONICET
Autor
Sosa, Andrea Mabel
Moya Alvarez, Agustin
Bracamonte, María Estefanía
Korenaga, Masataka
Marco, Jorge Diego
Barroso, Paola Andrea
Resumen
The treatment of leishmaniasis includes pentavalent antimony drugs but, because of the side effects, toxicity and cases of treatment failure or resistance, the search of new antileishmanial compounds are necessary. The aims of this study were to evaluate and compare the in vitro antileishmanial activity of four green tea catechins, and to assess the efficacy of topical (-)-epigallocatechin gallate in a cutaneous leishmaniasis model. The antileishmanial activity of green tea catechins was evaluated against intracellular amastigotes, and cytotoxicity was performed with human monocytic cell line. BALB/c mice were infected in the ear dermis with Leishmania (Leishmania) amazonensis and treated with topical 15% (-)-epigallocatechin gallate, intraperitoneal Glucantime, and control group. The efficacy of treatments was evaluated by quantifying the parasite burden and by measuring the lesions size. (-)-Epigallocatechin gallate and (-)-epigallocatechin were the most active compounds with IC50 values <59.6 μg/mL and with a selectivity index >1. Topical treatment with (-)-epigallocatechin gallate decreased significantly both lesion size and parasite burden (80.4% inhibition) compared to control group (p < 0.05), and moreover (-)-epigallocatechin gallate showed a similar efficacy to Glucantime (85.1% inhibition), the reference drug for leishmaniasis treatment.