info:eu-repo/semantics/article
Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells
Fecha
2018-01Registro en:
Prudkin Silva, Cecilia Raquel; Martinez, Jimena Hebe; Martínez, Karina Dafne; Farías Hermosilla, María Estefanía; Coluccio Leskow, Federico; et al.; Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells; Elsevier Science; Colloids and Surfaces A: Physicochemical and Engineering Aspects; 537; 1-2018; 425-434
0927-7757
CONICET Digital
CONICET
Autor
Prudkin Silva, Cecilia Raquel
Martinez, Jimena Hebe
Martínez, Karina Dafne
Farías Hermosilla, María Estefanía
Coluccio Leskow, Federico
Perez, Oscar Edgardo
Resumen
The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined and reproducible submicronic dimensions. I-CS complexes were obtained by mixing I and CS solutions at pH 2 and then increasing the pH up to 6 promoting electrostatic interactions between them. Colloidal stages of I and I-CS nano-complexes formation were characterized by dynamic light scattering (DLS), ζ-potential, solutions flow behavior and absorbance measurements. 1·10−2%, w/w, of CS allowed covering completely the surface protein aggregates constituting core–shell nano-structures of 200 nm, with a ζ-potential of 17,5 mV. Solution dynamic viscosity results kept relation with different stages of nano-complexation process. Biological activity of I-CS complexes was studied in 3T3-L1 cultured fibroblast showing a delayed and sustained activity as compared to free insulin. I-CS nano-complexes could be an alternative for developing a new generation of drugs allowing I protection from the hostile conditions of the body and increasing its absorption. These findings have basic and practical impacts as they could be exploited to exert the controlled release of I in therapeutic formulations by using the I-CS nano-complexes.