info:eu-repo/semantics/article
Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria
Fecha
2019-02Registro en:
Sabater, Carlos; Fara, María Agustina; Palacios, Jorge Martin; Corzo, Nieves; Requena, Teresa; et al.; Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria; Academic Press; Food Microbiology; 77; 2-2019; 93-105
0740-0020
1095-9998
CONICET Digital
CONICET
Autor
Sabater, Carlos
Fara, María Agustina
Palacios, Jorge Martin
Corzo, Nieves
Requena, Teresa
Montilla Corredera, Antonia
Zárate, Gabriela
Resumen
The potential of probiotic bacteria to produce prebiotic oligosaccharides by transgalactosylation has been minimally studied. In this work, we screened the β-galactosidase (β-gal) activity of dairy propionibacteria (PAB) isolated from Argentinean foods to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu). PAB, when grown in a medium with lactose as a carbon source, were disrupted, and the cell-free extracts were assayed for β-gal activity. Nine strains grew on lactose and showed β-gal activities from 0.27 to 2.60 U mL−1. Propionibacterium acidipropionici LET 120, the strain with the highest activity, was able to synthesize, using 30% lactose and lactulose at pH 6.5 and 45 °C, 26.8% of LET 120-GOS and 26.1% of LET 120-OsLu after 24 h. When they were tested as carbon sources for growth, P. acidipropionici LET 120 attained higher biomasses, μmax and β-gal activities at the expense of Aspergillus oryzae-OsLu, Vivinal®-GOS and lactulose compared to lactose or glucose. In addition, LET 120-GOS and LET 120-OsLu synthesized by PAB were prebiotic for some probiotic strains. For the first time, our results show the production of GOS and OsLu by dairy PAB, and these results encourage further studies on the optimization of the synthesis and structure characterization of the obtained oligosaccharides.