info:eu-repo/semantics/article
Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): Preliminary characterization
Fecha
2011-04-12Registro en:
Vannini, María Verónica; Rodríguez, Adriana; Vera, José Luis; de Valdéz, Graciela F.; Taranto, Maria Pia; et al.; Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): Preliminary characterization; Springer; Biotechnology Letters; 33; 8; 12-4-2011; 1625-1632
0141-5492
1573-6776
CONICET Digital
CONICET
Autor
Vannini, María Verónica
Rodríguez, Adriana
Vera, José Luis
de Valdéz, Graciela F.
Taranto, Maria Pia
Sesma, Fernando Juan Manuel
Resumen
Some strains of Lb. reuteri produce cobalamin (vitamin B12). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L-methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.