info:eu-repo/semantics/article
The Hepatitis E virus intraviral interactome
Fecha
2015-10Registro en:
Osterman, Andreas; Stellberger, Thorsten; Gebhardt, Anna; Kurz, M.arisa; Friedel, Caroline C.; et al.; The Hepatitis E virus intraviral interactome; Nature Publishing Group; Scientific Reports; 5; 13872; 10-2015; 1-13
2045-2322
CONICET Digital
CONICET
Autor
Osterman, Andreas
Stellberger, Thorsten
Gebhardt, Anna
Kurz, M.arisa
Friedel, Caroline C.
Uetz, Peter
Nitschko, Hans
Baiker, Armin
Vizoso Pinto, María Guadalupe
Resumen
Hepatitis E Virus (HEV) is an emerging virus causing epidemic acute hepatitis in developing countries as well as sporadic cases in industrialized countries. The life cycle of HEV is still poorly understood and the lack of efficient cell culture systems and animal models are the principal limitations for a detailed study of the viral replication cycle. Here we exhaustively examine all possible intraviral protein-protein interactions (PPIs) of HEV by systematic Yeast two-hybrid (Y2H) and LuMPIS screens, providing a basis for studying the function of these proteins in the viral replication cycle. Key PPIs correlate with the already published HEV 3D structure. Furthermore, we report 20 novel PPIs including the homodimerization of the RNA dependent RNA polymerase (RdRp), the self-interaction of the papain like protease, and ORF3 interactions with the papain-like protease and putative replicase components: RdRp, methylase and helicase. Furthermore, we determined the dissociation constant (Kd) of ORF3 interactions with the viral helicase, papain-like protease and methylase, which suggest a regulatory function for ORF3 in orchestrating the formation of the replicase complex. These interactions may represent new targets for antiviral drugs.