info:eu-repo/semantics/article
LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
Fecha
2009-12Registro en:
Vizoso Pinto, María Guadalupe; Villegas, Josefina Maria; Peter, Jan; Haase, Rudolf; Haas, Jürgen; et al.; LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs; Wiley VCH Verlag; Proteomics (weinheim. Print); 9; 23; 12-2009; 5303-5308
1615-9853
CONICET Digital
CONICET
Autor
Vizoso Pinto, María Guadalupe
Villegas, Josefina Maria
Peter, Jan
Haase, Rudolf
Haas, Jürgen
Lotz, Amelie Sophia
Muntau, Ania Carolina
Baiker, Armin
Resumen
The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
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