info:eu-repo/semantics/article
Bedaquiline and linezolid MIC distributions and epidemiological cut-off values for Mycobacterium tuberculosis in the Latin American region
Fecha
2019-02Registro en:
Lopez, Beatriz; Siqueira De Oliveira, Rosangela; Pinhata, Juliana M. W.; Chimara, Erica; Pacheco Ascencio, Edson; et al.; Bedaquiline and linezolid MIC distributions and epidemiological cut-off values for Mycobacterium tuberculosis in the Latin American region; Oxford University Press; Journal of Antimicrobial Chemotherapy; 74; 2; 2-2019; 373-379
0305-7453
CONICET Digital
CONICET
Autor
Lopez, Beatriz
Siqueira De Oliveira, Rosangela
Pinhata, Juliana M. W.
Chimara, Erica
Pacheco Ascencio, Edson
Puyén Guerra, Zully M.
Wainmayer, Ingrid
Símboli, Norberto Fabián
Del Granado, Mirtha
Palomino, Juan Carlos
Ritacco, Gloria Viviana
Martin, Anandi
Resumen
Objectives To describe the distributions of bedaquiline and linezolid MIC values for the Mycobacterium tuberculosis WT population and to define the corresponding epidemiological cut-offs (ECOFFs) in three Latin American countries. Methods MICs of bedaquiline and linezolid were determined by the resazurin microtitre assay (REMA). In phase 1, interlaboratory reproducibility was assessed using a panel of 10 fully susceptible M. tuberculosis strains. Phase 2 involved MIC determination for 248 clinical isolates from Argentina (n = 58), Brazil (n = 100) and Peru (n = 90) from patients who were treatment-naive for bedaquiline and linezolid. We then determined the ECOFFs for bedaquiline and linezolid by the eyeball method and the ECOFFinder statistical calculator. Results Phase 1: REMA MIC values in the three sites were either identical to each other or differed by one 2-fold dilution from the consensus value with the exception of a single value. Phase 2: The bedaquiline MIC range was 0.0039-0.25 mg/L for pan-susceptible and drug-resistant isolates combined. The linezolid MIC range was 0.062-0.5 mg/L for pan-susceptible isolates and 0.031-4 mg/L for drug-resistant isolates. ECOFFs were 0.125 mg/L for bedaquiline and 0.50 mg/L for linezolid. Conclusions REMA is reproducible and robust for the determination of bedaquiline and linezolid MIC distributions and ECOFF values when applied in laboratories of medium/low-resource countries. We suggest that WT MIC distributions for both drugs should be used as a monitoring tool to control the possible rapid emergence of resistance.