info:eu-repo/semantics/article
Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
Fecha
2010-03Registro en:
Mandal, Pinaki; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ghosh, Tuhin; Ray, Bimalendu; Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity; Elsevier Science; International Journal of Biological Macromolecules; 46; 2; 3-2010; 173-178
0141-8130
CONICET Digital
CONICET
Autor
Mandal, Pinaki
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Ghosh, Tuhin
Ray, Bimalendu
Resumen
Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V.