info:eu-repo/semantics/article
Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
Fecha
2016-01Registro en:
Vignoli, Rafael; García Fulgueiras, Virginia; Cordeiro, Nicolás F.; Bado, Inés; Seija, Verónica; et al.; Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 10; 1; 1-2016; 43-52
2036-6590
1972-2680
CONICET Digital
CONICET
Autor
Vignoli, Rafael
García Fulgueiras, Virginia
Cordeiro, Nicolás F.
Bado, Inés
Seija, Verónica
Aguerrebere, Paula
Laguna, Gabriel
Araújo, Lucía
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Chabalgoity Rodríguez, José Alejandro
Resumen
Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates. Results: Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9, or blaCTX-M-8 were found to be genetically unrelated. Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.