info:eu-repo/semantics/article
Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch)
Fecha
2019-06Registro en:
Langer, Silvia Estefanía; Marina, María; Burgos, Jose Luis; Martinez, Gustavo Adolfo; Civello, Pedro Marcos; et al.; Calcium chloride treatment modifies cell wall metabolism and activates defense responses in strawberry fruit (Fragaria × ananassa, Duch); John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 99; 8; 6-2019; 4003-4010
0022-5142
CONICET Digital
CONICET
Autor
Langer, Silvia Estefanía
Marina, María
Burgos, Jose Luis
Martinez, Gustavo Adolfo
Civello, Pedro Marcos
Villarreal, Natalia Marina
Resumen
BACKGROUND: Fruit dips in calcium ions solutions have been shown as an effective treatment to extend strawberries (Fragaria × ananassa, Duch) quality during storage. In the present work, strawberry fruit were treated with 10 g L−1 calcium chloride solution and treatment effects on cell wall enzymes activities and the expression of encoding genes, as well as enzymes involved in fruit defense responses were investigated. RESULTS: Calcium treatment enhanced pectin methylesterase activity while inhibited those corresponding to pectin hydrolases as polygalacturonase and β-galactosidase. The expression of key genes for strawberry pectin metabolism was up-regulated (for FaPME1) and down-regulated (for FaPG1, FaPLB, FaPLC, FaβGal1 and FaAra1) by calcium dips. In agreement, a higher firmness level and ionically-bound pectins (IBPs) amount were detected in calcium-treated fruit compared with controls. The in vitro and in vivo growth rate of fungal pathogen Botrytis cinerea was limited by calcium treatment. Moreover, the activities of polyphenol oxidases, chitinases, peroxidases and β-1,3-glucanases were enhanced by calcium ion dips. CONCLUSION: News insights concerning the biochemical and molecular basis of cell wall preservation and resistance to fungal pathogens on calcium-treated strawberries are provided.