dc.creator | Roldan, Julieta Suyay | |
dc.creator | Cassola, Alejandro Carlos | |
dc.creator | Castillo, Daniela Susana | |
dc.date.accessioned | 2021-09-08T17:05:40Z | |
dc.date.accessioned | 2022-10-15T12:23:33Z | |
dc.date.available | 2021-09-08T17:05:40Z | |
dc.date.available | 2022-10-15T12:23:33Z | |
dc.date.created | 2021-09-08T17:05:40Z | |
dc.date.issued | 2020-03 | |
dc.identifier | Roldan, Julieta Suyay; Cassola, Alejandro Carlos; Castillo, Daniela Susana; Optimization of recombinant Zika virus NS1 protein secretion from HEK293 cells; Elsevier; Biotechnology Reports; 25; 3-2020; 1-10 | |
dc.identifier | 2215-017X | |
dc.identifier | http://hdl.handle.net/11336/139915 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4385597 | |
dc.description.abstract | Sensitive, accurate and cost-effective diagnostic tests are urgently needed to detect Zika virus (ZIKV) infection. Nonstructural 1 (NS1) glycoprotein is an excellent diagnostic marker since it is released in a hexameric conformation from infected cells into the patient's bloodstream early in the course of the infection. We established a stable rZNS1-His-expression system in HEK293 cells through lentiviral transduction. A novel optimization approach to enhance rZNS1-His protein secretion in the mammalian expression system was accomplished through 50 nM rapamycin incubation followed by serum-free media incubation for 9 days, reaching protein yields of ∼10 mg/l of culture medium. Purified rZNS1-His hexamer was recognized by anti-NS1 antibodies in ZIKV patient's serum, and showed the ability to induce a humoral response in immunized mice. The obtained recombinant protein is a reliable biological tool that can potentially be applied in the development of diagnostic tests to detect ZIKV in infected patients during the acute phase. | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2215017X19306228 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.btre.2020.e00434 | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | MAMMALIAN EXPRESSION SYSTEM | |
dc.subject | NS1 | |
dc.subject | RECOMBINANT PROTEIN | |
dc.subject | ZIKA VIRUS | |
dc.title | Optimization of recombinant Zika virus NS1 protein secretion from HEK293 cells | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |