dc.creatorAguilera, Milton Osmar
dc.creatorBeron, Walter
dc.creatorColombo, María Isabel
dc.date.accessioned2021-10-25T18:54:02Z
dc.date.accessioned2022-10-15T12:19:16Z
dc.date.available2021-10-25T18:54:02Z
dc.date.available2022-10-15T12:19:16Z
dc.date.created2021-10-25T18:54:02Z
dc.date.issued2012-08-06
dc.identifierAguilera, Milton Osmar; Beron, Walter; Colombo, María Isabel; The actin cytoskeleton participates in the early events of autophagosome formation upon starvation induced autophagy; Landes Bioscience; Autophagy; 8; 11; 6-8-2012; 1590-1603
dc.identifier1554-8627
dc.identifierhttp://hdl.handle.net/11336/144989
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4385193
dc.description.abstractAutophagy is a process by which cytoplasmic material is sequestered in a double-membrane vesicle destined for degradation. Nutrient deprivation stimulates the pathway and the number of autophagosomes in the cell increases in response to such stimulus. In the current report we have demonstrated that actin is necessary for starvationmediated autophagy. When the actin cytoskeleton is depolymerized, the increase in autophagic vacuoles in response to the starvation stimulus was abolished without affecting maturation of remaining autophagosomes. In addition, actin filaments colocalized with ATG14, BEC N1/Beclin1 and PtdIns3P-rich structures, and some of them have a typical omegasome shape stained with the double FYVE domain or ZFYVE1/DFCP1. In contrast, no major colocalization between actin and ULK1, ULK2, ATG5 or MAP1LC3/LC3 was observed. Taken together, our data indicate that actin has a role at very early stages of autophagosome formation linked to the PtdIns3P generation step. In addition, we have found that two members of the Rho family of proteins, RHOA and RAC1 have a regulatory function on starvation-mediated autophagy, but with opposite roles. Indeed, RHOA has an activatory role whereas Rac has an inhibitory one. We have also found that inhibition of the RHOA effector ROCK impaired the starvation-mediated autophagic response. We propose that actin participates in the initial membrane remodeling stage when cells require an enhanced rate of autophagosome formation, and this actin function would be tightly regulated by different members of the Rho family.
dc.languageeng
dc.publisherLandes Bioscience
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4161/auto.21459
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.4161/auto.21459
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectACTIN
dc.subjectAUTOPHAGOSOME FORMATION
dc.subjectRAC1
dc.subjectRHOA
dc.subjectROCK
dc.subjectSTARVATION
dc.titleThe actin cytoskeleton participates in the early events of autophagosome formation upon starvation induced autophagy
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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