info:eu-repo/semantics/article
Immune response to Neospora caninum live tachyzoites in prepubertal female calves
Fecha
2019-10Registro en:
Hecker, Yanina Paola; Regidor Cerrillo, Javier; Fiorani, Franco; Horcajo, Pilar; Soria, Ivana; et al.; Immune response to Neospora caninum live tachyzoites in prepubertal female calves; Springer; Parasitology Research; 118; 10; 10-2019; 2945-2955
0932-0113
CONICET Digital
CONICET
Autor
Hecker, Yanina Paola
Regidor Cerrillo, Javier
Fiorani, Franco
Horcajo, Pilar
Soria, Ivana
Gual, Ignacio
Torioni, Susana
Campero, Lucía María
Echaide, Ignacio Eduardo
Álvarez García, Gema
Ortega Mora, Luis Miguel
Zamorano, Patricia Ines
Venturini, María C.
Odeón, Anselmo Carlos
Cantón, Germán J.
Moore, Dadin Prando
Resumen
The aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly allocated into two groups: group A calves were inoculated subcutaneously (sc) with 1 × 106 tachyzoites of the low virulence NC-Argentina LP1 isolate in sterile phosphate-buffered saline (PBS); group B calves were mock inoculated sc with sterile PBS. Calves from group A developed a specific immune response characterized by the production of IgG antibodies and the expression of IFN-γ and TNF-α cytokines. Animals did not present any febrile reaction or reactions at the site of inoculation. Although chronic N. caninum infection was developed in 50% of calves of group A after inoculation, according to the presence of antibodies against rNc-SAG4, antigen characteristic of bradyzoites, N. caninum antibodies dropped below the cut-off of ELISA from day 210 post-inoculation onwards. Future trials using the same group of inoculated animals will allow the characterization of the evolution of the immune response during pregnancy and to determine whether the immunization with the local isolate is able to prevent congenital transmission and to protect against heterologous challenges.