info:eu-repo/semantics/article
Detection of citrus psorosis virus by RT-qPCR validated by diagnostic parameters
Fecha
2021-05Registro en:
Simeone, Melina Ailin; Gómez, Claudio; Bertalmío, Ana; Ruiz, María Esperanza; Hauteville, Claudia; et al.; Detection of citrus psorosis virus by RT-qPCR validated by diagnostic parameters; Wiley Blackwell Publishing, Inc; Plant Pathology; 70; 4; 5-2021; 980-986
0032-0862
1365-3059
CONICET Digital
CONICET
Autor
Simeone, Melina Ailin
Gómez, Claudio
Bertalmío, Ana
Ruiz, María Esperanza
Hauteville, Claudia
Godoy Suarez, Laura
Tito, Blas Alfredo
Garcia, Maria Laura
Resumen
Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft-transmissible diseases in many citrus-growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT-qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT-qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT-qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees.