info:eu-repo/semantics/article
Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
Fecha
2017-06-20Registro en:
Scarpeci, Telma Eleonora; Zanor, María Inés; Valle, Estela Marta; Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints; Bio-protocol LLC; Bio-protocol; 7; 12; 20-6-2017; 1-6
2331-8325
CONICET Digital
CONICET
Autor
Scarpeci, Telma Eleonora
Zanor, María Inés
Valle, Estela Marta
Resumen
Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With this technique, it is possible to get the real picture at any moment. The outputs of the data include studies on cell area and morphology, epidermis cell and stomata lineages, among others. This protocol is useful for the accurate estimation of stomatal aperture in many samples of intact leaves in Arabidopsis thaliana.