dc.creatorVidal, Ezequiel Martin
dc.creatorPalomeque, Miriam Edid
dc.creatorLista, Adriana Guillermina
dc.creatorFernández Band, Beatriz Susana
dc.date.accessioned2020-02-07T19:32:09Z
dc.date.accessioned2022-10-15T11:20:37Z
dc.date.available2020-02-07T19:32:09Z
dc.date.available2022-10-15T11:20:37Z
dc.date.created2020-02-07T19:32:09Z
dc.date.issued2003-12
dc.identifierVidal, Ezequiel Martin; Palomeque, Miriam Edid; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Flow injection analysis: Rayleigh light scattering technique for total protein determination; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 376; 1; 12-2003; 38-41
dc.identifier1618-2642
dc.identifierhttp://hdl.handle.net/11336/96922
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4380159
dc.description.abstractA novel flow injection analysis (FIA) method with Rayleigh light scattering (RLS) detection was developed for the determination of total protein concentrations. This method is based on the weak intensity of RLS of bromothymol blue (BB) (3′,3″-dibromothymolsulfonephthalein) which can be enhanced by the addition of protein in weakly acidic solution. A common spectrofluorimeter was used as a detector. It was proved that the application of this method to quantify the total proteins in real samples by using bovine serum albumin was possible. The RLS signal was detected at λ ex=λ em=572 nm. The linear range was 7.0-70.0 μg mL-1, the detection limit was 3.75 μg mL-1, the reproducibility was 5.5% (n=7), and the sample throughput was 26 h-1.
dc.languageeng
dc.publisherSpringer Heidelberg
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00216-003-1877-2
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s00216-003-1877-2
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectFIA
dc.subjectPROTEINS
dc.subjectRAYLEIGH LIGHT SCATTERING
dc.titleFlow injection analysis: Rayleigh light scattering technique for total protein determination
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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