info:eu-repo/semantics/article
Synthetic and biological identities of layered double hydroxides nanocarriers functionalized with risedronate
Fecha
2020-12-01Registro en:
Vasti, Cecilia; Aristizabal, Dariana; Bonnet, Laura Vanesa; Ambroggio, Ernesto Esteban; Giacomelli, Carla Eugenia; et al.; Synthetic and biological identities of layered double hydroxides nanocarriers functionalized with risedronate; Elsevier Science; Applied Clay Science; 199; 1-12-2020; 1-10;105880
0169-1317
CONICET Digital
CONICET
Autor
Vasti, Cecilia
Aristizabal, Dariana
Bonnet, Laura Vanesa
Ambroggio, Ernesto Esteban
Giacomelli, Carla Eugenia
Rojas Delgado, Ricardo
Resumen
Layered double hydroxide nanoparticles (LDH-NP) provide new therapeutic opportunities to improve drug distribution, stability, and effectivity. Nevertheless, these LDH-NP present a different behavior in biological medium due to their interaction with biomolecules (biological identity) to that of as-synthesized LDH-NP (synthetic identity). In this work, both identities were compared for LDH-NP functionalized with risedronate. First, physical interactions between as-synthesized LDH-NP and the cell membrane were studied using giant unilamellar vesicles (GUVs). Then, their biological identity was formed in bovine fetal serum and explored with a proteomic approach and the mapping ofprotein-protein interactions. Finally, the effect of functionalization and biological identity on drug delivery capacity was tested in methotrexate loaded LDH-NP. As a result of their opposite zeta potential, bare LDH-NP (ζ > +40 mV) increased the permeability of the negatively charged GUVs, while Ris functionalized LDH-NP (ζ < −40 mV) showed a much lower permeability increase. Also, a heavily interconnected protein corona with more than 70 different proteins was formed on bare LDH-NP while the number and interconnection of the proteins in the corona were diminished on Ris functionalized ones. Also, Ris functionalization only slightly diminished the drug loading capacity ofthe nanocarriers, which nevertheless showed a significant cytotoxic effect associated to the ntercalated drug.