info:eu-repo/semantics/article
Immobilization of bacteria in microgel grafted onto macroporous polyethylene
Fecha
2010-03Registro en:
Trelles, Jorge Abel; Quiroga, Flavia Yanina; Britos, Claudia Noelia; Smolko, Eduardo E.; Grasselli, Mariano; Immobilization of bacteria in microgel grafted onto macroporous polyethylene; Pergamon-Elsevier Science Ltd; Radiation Physics and Chemistry (Oxford); 79; 3; 3-2010; 241-245
0969-806X
CONICET Digital
CONICET
Autor
Trelles, Jorge Abel
Quiroga, Flavia Yanina
Britos, Claudia Noelia
Smolko, Eduardo E.
Grasselli, Mariano
Resumen
The development of "Green Chemistry" requires new materials to replace the conventional organic chemistry by biological catalysts, to produce fine chemicals in an environmentally friendly manner. Microbial whole cells can be directly used as biocatalysts, providing a simple and cheap methodology since enzyme isolation and purification are avoided. High-density polyethylene (HDPE) is a very stable polymer though it can be activated by gamma radiation to induce grafting. Glycidyl methacrylate was grafted onto macroporous HDPE and PP in the range of 1-6%, proportional to the initial monomer concentration. Grafted polymers were further chemically modified with ethylenediamine to generate a cationic hydrogel of micron-size thickness onto the internal polymer surfaces. Modified polymers were able to immobilize Gram-positive and Gram-negative bacteria that can catalyze a chemical reaction as efficient as free cells do.