info:eu-repo/semantics/article
Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade
Fecha
2020-05Registro en:
Romanutti, Carina; Keller, Leticia; La Torre, José; Panzera, Yanina; Fuques, Eddie; et al.; Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade; Elsevier Science; Journal of Virological Methods; 279; 113857; 5-2020; 1-7
0166-0934
CONICET Digital
CONICET
Autor
Romanutti, Carina
Keller, Leticia
La Torre, José
Panzera, Yanina
Fuques, Eddie
Pérez, Ruben
Gallo Calderon, Marina Beatriz
Resumen
Canine Distemper Virus (CDV) is a highly contagious pathogen of dogs that causes severe respiratory, gastrointestinal and nervous signs. Although vaccines have been used to prevent infections, CDV has been reported worldwide, even in vaccinated animals. In the present study, a representative wild type CDV strain (Arg24) was isolated from a sick vaccinated dog and its genome was completely sequenced using Illumina technology. This strain produced a strong cytopathic effect in Vero SLAM (Signaling Lymphocyte Activation Molecule) cells with a higher titer of 1.1 × 105 Median Tissue Culture Infectious Dose (TCID50/mL) at 32 h post infection, in cell-associated virus. The Arg24 strain genome, showed values of 97.1, 90.3, 96.7, 90.6, 89.8 and 97.3 % of amino acid identity with respect to the Onderstepoort vaccine strain (Nucleoprotein, Phosphoprotein, Matrix, Fusion, Hemagglutinin and Large polymerase, respectively). Focusing on the Hemagglutinin gene, which is the target for genetic characterization, Arg24 showed four additional potential glycosylation sites, with respect to the Onderstepoort. The availability of Arg24 strain, which can be easily grown in Vero SLAM cells, is an important tool to perform immunological and antigenic comparative studies, between wild type and vaccine CDV strains.