info:eu-repo/semantics/article
Nicotinic acetylcholine receptors at the single-channel level
Fecha
2018-06Registro en:
Bouzat, Cecilia Beatriz; Sine, Steven M.; Nicotinic acetylcholine receptors at the single-channel level; Wiley Blackwell Publishing, Inc; British Journal of Pharmacology; 175; 11; 6-2018; 1789-1804
0007-1188
1476-5381
CONICET Digital
CONICET
Autor
Bouzat, Cecilia Beatriz
Sine, Steven M.
Resumen
Over the past four decades, the patch clamp technique and nicotinic ACh (nACh) receptors have established an enduring partnership. Like all good partnerships, each partner has proven significant in its own right, while their union has spurred innumerable advances in life science research. A member and prototype of the superfamily of pentameric ligand-gated ion channels, the nACh receptor is a chemo-electric transducer, binding ACh released from nerves and rapidly opening its channel to cation flow to elicit cellular excitation. A subject of a Nobel Prize in Physiology or Medicine, the patch clamp technique provides unprecedented resolution of currents through single ion channels in their native cellular environments. Here, focusing on muscle and α7 nACh receptors, we describe the extraordinary contribution of the patch clamp technique towards understanding how they activate in response to neurotransmitter, how subtle structural and mechanistic differences among nACh receptor subtypes translate into significant physiological differences, and how nACh receptors are being exploited as therapeutic drug targets.