info:eu-repo/semantics/article
Biotransformation of cladribine by a magnetic immobilizated biocatalyst of Lactobacillus animalis
Fecha
2020-07Registro en:
Lapasset Laumann, Aldana Stephanie; Britos, Claudia Noelia; Cappa, Valeria Alejandra; Rivero, Cintia Wanda; Trelles, Jorge Abel; Biotransformation of cladribine by a magnetic immobilizated biocatalyst of Lactobacillus animalis; Springer; Biotechnology Letters; 42; 7; 7-2020; 1229-1236
0141-5492
1573-6776
CONICET Digital
CONICET
Autor
Lapasset Laumann, Aldana Stephanie
Britos, Claudia Noelia
Cappa, Valeria Alejandra
Rivero, Cintia Wanda
Trelles, Jorge Abel
Resumen
A stable biocatalyst with magnetic properties based on immobilized Lactobacillus animalis ATCC 35,046 to obtain 2-chloroadenine-2′-deoxyriboside, known as cladribine, is reported for the first time. This nucleoside analogue is an antitumor agent used in the treatment of a wide variety of types of leukemia. In this study, an eco-compatible and alternative bioprocess to obtain cladribine was developed. Product conversion was close to 90% at 2 h in optimized nonconventional reaction media. The microscale biosynthesis of the compound of interest afforded a total productivity close to 370 mg/L/h in the presence of DMSO, and it was stable at least for 30 days in storage conditions.