info:eu-repo/semantics/article
Enhanced gold nanoparticle-tumor cell recognition by albumin multilayer coating
Fecha
2021-12Registro en:
Achilli, Estefanía Edith; Flores, Constanza Yanel; Temprana, Carlos Facundo; Alonso, Silvia del Valle; Radrizzani Helguera, Martin; et al.; Enhanced gold nanoparticle-tumor cell recognition by albumin multilayer coating; Elsevier; OpenNano; 2021; 12-2021; 1-12
2352-9520
CONICET Digital
CONICET
Autor
Achilli, Estefanía Edith
Flores, Constanza Yanel
Temprana, Carlos Facundo
Alonso, Silvia del Valle
Radrizzani Helguera, Martin
Grasselli, Mariano
Resumen
Background: In a biological environment, nanoparticles are rapidly coated with serum proteins, which affects the NPs transport through biological medium, cellular uptake and response, all of which can impair therapeutic efficiency. Reduction of non-specific adsorption of proteins is mandatory to overcome this drawback.
Aim: We propose to use albumin to prepare a multilayer coating of NPs to reduce the non-specific protein interactions in biological media.
Materials & methods: Biohybrid NPs (bioHNPs) prepared by coating gold NPs with a multilayer of albumin and finally decorated with Bombesin-related peptides (BD-bioHNPs).
Results: BioHNPs/biological media interaction was characterized by physicochemical and biological techniques under near-physiological conditions. A significant reduction of the Corona effect and enhanced in vitro uptake to PC-3 cells was demonstrated for BD-bioHNPs.
Conclusion: This methodology to prepare decorated bioHNPs allows the preparation of ‘stealth’ NPs with improved cell targeting and the ability to avoid non-specific interactions with the biological media.