info:eu-repo/semantics/publishedVersion
Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
Fecha
2014Registro en:
Romero, Marcelo Ricardo; Slenk, Walter; Montero, Felipe; Juárez Data, Roger Martín Noé; Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step; GRIN Publishing GmbH; 1; 2014; 5
9783656846154
CONICET Digital
CONICET
Autor
Romero, Marcelo Ricardo
Slenk, Walter
Montero, Felipe
Juárez Data, Roger Martín Noé
Resumen
A synthesis of IMAC adsorbent was performed. Agarose matrix was derivatized with epichlorohydrin using different amounts of NaBH4. Furthermore, iminodiacetic acid was immobilized on the modified agarose. Finally, the derivatization efficiency was studied by measuring the epoxide groups and retention of Cu2+. The results indicated that the matrices were modified efficiently and the use of NaBH4 in small amounts is enough to provide a reducing environment to the reaction.