info:eu-repo/semantics/article
Optical and Thermophoretic Control of Janus Nanopen Injection into Living Cells
Fecha
2018-12Registro en:
Maier, Christoph M.; Huergo, María Ana Cristina; Milosevic, Sara; Pernpeintner, Carla; Li, Miao; et al.; Optical and Thermophoretic Control of Janus Nanopen Injection into Living Cells; American Chemical Society; Nano Letters; 18; 12; 12-2018; 7935-7941
1530-6984
CONICET Digital
CONICET
Autor
Maier, Christoph M.
Huergo, María Ana Cristina
Milosevic, Sara
Pernpeintner, Carla
Li, Miao
Singh, Dhruv P.
Walker, Debora
Fischer, Peer
Feldmann, Jochen
Lohmüller, Theobald
Resumen
Devising strategies for the controlled injection of functional nanoparticles and reagents into living cells paves the way for novel applications in nanosurgery, sensing, and drug delivery. Here, we demonstrate the light-controlled guiding and injection of plasmonic Janus nanopens into living cells. The pens are made of a gold nanoparticle attached to a dielectric alumina shaft. Balancing optical and thermophoretic forces in an optical tweezer allows single Janus nanopens to be trapped and positioned on the surface of living cells. While the optical injection process involves strong heating of the plasmonic side, the temperature of the alumina stays significantly lower, thus allowing the functionalization with fluorescently labeled, single-stranded DNA and, hence, the spatially controlled injection of genetic material with an untethered nanocarrier.