info:eu-repo/semantics/article
Human papillomavirus DNA detection and typing in male urine samples from a high-risk population from Argentina
Fecha
2005-03Registro en:
Golijow, Carlos Daniel; Perez, Luis Orlando; Smith, Jennifer S.; Abba, Martín Carlos; Human papillomavirus DNA detection and typing in male urine samples from a high-risk population from Argentina; Elsevier Science; Journal of Virological Methods; 124; 1-2; 3-2005; 217-220
0166-0934
CONICET Digital
CONICET
Autor
Golijow, Carlos Daniel
Perez, Luis Orlando
Smith, Jennifer S.
Abba, Martín Carlos
Resumen
The aim of the present study was to evaluate the first void urine (FVU) as a non-invasive sampling method for HPV detection and genotyping in a high-risk population. Men presenting with HPV associated penile lesions or HPV positive partners attending a urological department in La Plata, Argentina were enrolled for HPV detection and genotyping. DNA from 185 first-void urine samples was evaluated for the presence of HPV by nested polymerase chain reaction using MY09/11 and GP05/06 primers. The viral genotype was analyzed by means of the single-stranded conformation polymorphisms (SSCP) method. Seventy-three percent (135/185) of the FVU specimens were positive for HPV-DNA. The viral prevalence in patients with HPV-DNA positive partners was 68.8% (77/112), and 79.5% (58/73) of patients with penile lesions were found to be HPV positive. The most frequent viral type was HPV-11 (26.7%), followed by HPV-6 (23%), HPV-16 (21.5%), HPV-18 (6%), and HPV-31 (4.4%). In this study, 11.1% (15/135) of the HPV positive specimens were double infections. These results indicate that high-risk HPVs can be found in clinical lesions in a high percentage (43.8%), as simple or double infections. In this sense, the male population represents an important reservoir for the virus and may play a role in the transmission and perpetuation of the infection in the general population. The method described below provides a tool for detection and typing of HPV-DNA using samples obtained by non-invasive techniques and thus easy to obtain.