info:eu-repo/semantics/article
Exposure of neonatal female rats to bisphenol A disrupts hypothalamic LHRH pre-mRNA processing and estrogen receptor alpha expression in nuclei controlling estrous cyclicity
Fecha
2010-12Registro en:
Monje, Lucas Daniel; Varayoud, Jorgelina Guadalupe; Muñoz de Toro, Monica Milagros; Luque, Enrique Hugo; Ramos, Jorge Guillermo; Exposure of neonatal female rats to bisphenol A disrupts hypothalamic LHRH pre-mRNA processing and estrogen receptor alpha expression in nuclei controlling estrous cyclicity; Elsevier Science; Reproductive Toxicology; 30; 4; 12-2010; 625-634
0890-6238
CONICET Digital
CONICET
Autor
Monje, Lucas Daniel
Varayoud, Jorgelina Guadalupe
Muñoz de Toro, Monica Milagros
Luque, Enrique Hugo
Ramos, Jorge Guillermo
Resumen
This study examines the effects of neonatal exposure to the endocrine disruptor bisphenol A (BPA) on the neural network that controls estrous cyclicity. From postnatal day 1 (PND1) to PND7, female pups were injected with vehicle (control) or BPA (BPA.05: 0.05. mg/kg-d, BPA20: 20. mg/kg-d). At PND100 BPA.05-females showed alterations in estrous cyclicity and BPA20-females were incapable of producing an estradiol-induced LH surge. By real-time PCR we determined that hypothalamic expression of mature LH-releasing hormone (LHRH) mRNA was increased in BPA.05 and decreased in BPA20-females. Furthermore, unprocessed intron A-containing LHRH RNA was decreased in the cytoplasm of hypothalamic cells of both groups. Immunohistochemistry revealed that estrogen receptor alpha protein was up-regulated in anteroventral periventricular and down-regulated in arcuate nucleus of both groups. Our results show that BPA permanently disrupts hypothalamic LHRH pre-mRNA processing and steroid receptors expression in nuclei that control estrous cyclicity in adult rats.