info:eu-repo/semantics/publishedVersion
Whole cell patch clamp recording of mouse and rat inner hair cells in the intact organ of Corti
Fecha
2016Registro en:
Goutman, Juan Diego; Pyott, Sonja J.; Whole cell patch clamp recording of mouse and rat inner hair cells in the intact organ of Corti; Springer; 1427; 2016; 471-485
978-1-59745-523-7
CONICET Digital
CONICET
Autor
Goutman, Juan Diego
Pyott, Sonja J.
Resumen
Whole cell patch clamping is a widely applied method to record currents across the entire membrane of a cell. This protocol describes application of this method to record currents from the sensory inner hair cells in the intact auditory sensory epithelium, the organ of Corti, isolated from rats or mice. This protocol particularly outlines the basic equipment required, provides instructions for the preparation of solutions and small equipment items, and methodology for recording voltage-activated and evoked synaptic currents from the inner hair cells.