info:eu-repo/semantics/article
Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition
Fecha
2020-02Registro en:
Ale, Elisa Carmen; Batistela, Virginia Alejandra; Correa Olivar, Gabriela Alejandra; Ferrado, Joana Belén; Sadiq, Sohaib; et al.; Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition; Wiley Blackwell Publishing, Inc; International Journal Of Dairy Technology; 73; 1; 2-2020; 76-87
1364-727X
CONICET Digital
CONICET
Autor
Ale, Elisa Carmen
Batistela, Virginia Alejandra
Correa Olivar, Gabriela Alejandra
Ferrado, Joana Belén
Sadiq, Sohaib
Ahmed, Hafiz I.
Reinheimer, Jorge Alberto
Vera Candioti, Luciana
Laws, Andrew P.
Binetti, Ana Griselda
Resumen
Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β-glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semidefined medium (SDM, 0.63% yeast nitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterisation indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β-glucan.