info:eu-repo/semantics/article
Detecting phospholipase activity with the amphipathic lipid packing sensor motif of ArfGAP1
Fecha
2018-10Registro en:
Yunes Quartino, Pablo Javier; Fidelio, Gerardo Daniel; Manneville, Jean Baptiste; Goud, Bruno; Ambroggio, Ernesto Esteban; Detecting phospholipase activity with the amphipathic lipid packing sensor motif of ArfGAP1; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 505; 1; 10-2018; 290-294
0006-291X
CONICET Digital
CONICET
Autor
Yunes Quartino, Pablo Javier
Fidelio, Gerardo Daniel
Manneville, Jean Baptiste
Goud, Bruno
Ambroggio, Ernesto Esteban
Resumen
The amphipathic lipid packing sensor (ALPS) motif of ArfGAP1 brings this GTPase activating protein to membranes of high curvature. Phospholipases are phospholipid-hydrolyzing enzymes that generate different lipid products that alter the lateral organization of membranes. Here, we evaluate by fluorescence microscopy how in-situ changes of membrane lipid composition driven by the activity of different phospholipases promotes the binding of ALPS. We show that the activity of phospholipase A2, phospholipase C and phospholipase D drastically enhances the binding of ALPS to the weakly-curved membrane of giant liposomes. Our results suggest that the enzymatic activity of phospholipases can modulate the ArfGAP1-mediated intracellular traffic and that amphiphilic peptides such as the ALPS motif can be used to study lipolytic activities at lipid membranes.