info:eu-repo/semantics/article
In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates
Fecha
2019-10Registro en:
Rodrigues, Mariana Paiva; Astoreca, Andrea Luciana; De Oliveira, Águida Aparecida; Salvato, Lauranne Alves; Biscoto, Gabriela Lago; et al.; In vitro activity of neem (Azadirachta indica) oil on growth and ochratoxin A production by Aspergillus carbonarius isolates; Multidisciplinary Digital Publishing Institute; Toxins; 11; 10; 10-2019; 1-12
2072-6651
CONICET Digital
CONICET
Autor
Rodrigues, Mariana Paiva
Astoreca, Andrea Luciana
De Oliveira, Águida Aparecida
Salvato, Lauranne Alves
Biscoto, Gabriela Lago
Keller, Luiz Antonio Moura
Rosa, Carlos Alberto da Rocha
Cavaglieri, Lilia Reneé
De Azevedo, Maria Isabel
Keller, Kelly Moura
Resumen
Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.