info:eu-repo/semantics/article
Spin label EPR suggests the presence of cholesterol rich domains in cultured insect cell membranes
Fecha
2018-11Registro en:
Baque, Maria Alejandra; Gioria, Verónica Viviana; Micheloud, Gabriela Analia; Casado, Nieves Maria Claudia; Claus, Juan Daniel; et al.; Spin label EPR suggests the presence of cholesterol rich domains in cultured insect cell membranes; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 505; 4; 11-2018; 1038-1042
0006-291X
CONICET Digital
CONICET
Autor
Baque, Maria Alejandra
Gioria, Verónica Viviana
Micheloud, Gabriela Analia
Casado, Nieves Maria Claudia
Claus, Juan Daniel
Gennaro, Ana Maria
Resumen
Different spin labels were incorporated to the membranes of cultured insect UFL-AG-286 cells in order to characterize their physical properties by Electron Paramagnetic Resonance spectroscopy (EPR). The spectrum of the spin label 12-SASL incorporated to cell membranes was similar as those obtained in membrane model systems composed of eggPC/cholesterol. However, the spectrum of the spin label CSL, chemically related to cholesterol, was drastically different in the two systems. Interestingly, when cell cholesterol content was reduced using methyl beta cyclodextrin, an EPR spectrum similar to those of model membranes was obtained. The analysis of these experiments suggests the existence of cholesterol rich regions in UFL-AG-286 cell membranes.