info:eu-repo/semantics/article
In vitro propagation and stoloniferous stem formation of Thelesperma megapotamicum
Fecha
2010-04Registro en:
Figueroa, A. C.; Diaz, S.; Palacio, Lorena; Goleniowski, Marta Ester; In vitro propagation and stoloniferous stem formation of Thelesperma megapotamicum; Instituto de Investigación de las Ciencias Exactas Físicas y Naturales; Molecular Medicinal Chemistry; 21; 4-2010; 127-131
1666-888X
CONICET Digital
CONICET
Autor
Figueroa, A. C.
Diaz, S.
Palacio, Lorena
Goleniowski, Marta Ester
Resumen
An efficient “in vitro” propagation protocol for Thelesperma megapotamicum (Spreng.) Kuntnze was established. Nodal segments were cultured in vitro with different combination and concentration of growth regulators. Microshoots (60-days) cultured in Murashige and Skoog (MS) with the addition of 4 mg/l 6-benzyl adenine (BA) were used as explants and incubated on a half salt concentrations MS/2 medium supplemented with different combination of indol butyric acid (IBA) and sucrose for 120 days. Explants cultured on a half of Murashige and Skoog salts (MS/2) with the addition of 1,0 mg/L-40 g/L and 1,5mg/L - 30g/L of IBA and sucrose gave shoot number and principal stem length as the control medium with higher stoloniferous stem percentage or development of shoots in stoloniferous stem respectively.