info:eu-repo/semantics/article
Nanocompartmentalization of the Nuclear Pore Lumen
Fecha
2020-05Registro en:
Huang, Kai; Tagliazucchi, Mario Eugenio; Park, Sung Hyun; Rabin, Yitzhak; Szleifer, Igal; Nanocompartmentalization of the Nuclear Pore Lumen; Cell Press; Biophysical Journal; 118; 1; 5-2020; 219-231
0006-3495
CONICET Digital
CONICET
Autor
Huang, Kai
Tagliazucchi, Mario Eugenio
Park, Sung Hyun
Rabin, Yitzhak
Szleifer, Igal
Resumen
The nuclear pore complex (NPC) employs the intrinsically disordered regions (IDRs) from a family of phenylalanine-glycine-rich nucleoporins (FG-Nups) to control nucleocytoplasmic transport. It has been a long-standing mystery how the IDR-mediated mass exchange can be rapid yet selective. Here, we use a computational microscope to show that nanocompartmentalization of IDR subdomains leads to a remarkably elaborate gating structure as programmed by the amino acid sequences. In particular, we reveal a heterogeneous permeability barrier that combines an inner ring barrier with two vestibular condensates. Throughout the NPC, we find a polarized electrostatic potential and a diffuse thermoreversible FG network featuring mosaic FG territories with low FG-FG pairing fraction. Our theoretical anatomy of the central transporter sheds light into the sequence-structure-function relationship of the FG-Nups and provides a picture of nucleocytoplasmic mass exchange that allows a reconciliation of transport efficiency and specificity.